Journal: Photochemistry and photobiology

Article Title: The B 6 -vitamer Pyridoxal is a Sensitizer of UVA-induced Genotoxic Stress in Human Primary Keratinocytes and Reconstructed Epidermis †

doi: 10.1111/php.12720

Figure Lengend Snippet: After exposure to the isolated or combined action of UVA (6.6 J/cm2) and PL (250 μM), cells were rinsed and then cultured in medium (6 h) followed by Stress and Toxicity Pathfinder RT2Profiler™ PCR Expression array analysis. Scatter blots depict differential gene expression (cut-off lines: threefold up- or down-regulation). Arrays were performed in three independent repeats and analyzed using the two-sided Student’s t test. (A) PL-induced gene expression (versus untreated). (B) UVA-induced gene expression (versus untreated). (C) PL/UVA-induced gene expression (versus untreated). (D) Numerical expression changes (PL/UVA versus untreated) [n=3, mean ± SD; (p<0.05)].

Article Snippet: The Human Stress and Toxicity Pathfinder RT 2 ProfilerTM PCR Expression Array (SuperArray) profiling the expression of 84 stress-related genes was employed as described before ( 30 ).

Techniques: Isolation, Cell Culture, Expressing, Gene Expression

Journal: Human Molecular Genetics

Article Title: Functional genomic analysis unravels a metabolic-inflammatory interplay in adrenoleukodystrophy

doi: 10.1093/hmg/ddr536

Figure Lengend Snippet: Activation of TLR/NF-κB in mouse spinal cords at 12 months of age. A q-PCR was performed to measure a gene array including TLRs, TLR adaptors, NF-κB forms, several cytokines and their receptors. Only differentially expressed genes are shown ( n = 6 individuals per genotype). The data point to a general activation of TLR/NF-κB pathways, wherein we highlight the upregulation of Tlr8 , Tlr5 , Nfkbib , RANTES ( Ccl5 ), Ccr1 , Ccr2 , Ccr7 , Il1b , Tnf and Lta . Significant differences have been determined by Student's t -test: * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: PCR microarray for TLR pathway was performed by using RT2-Profiler PCR Array from SuperArray Bioscience (Mouse Toll-Like Receptor Signalling Pathway PAMM-018 and Inflammatory Cytokines and Receptors PAMM-011).

Techniques: Activation Assay

Journal: Nature immunology

Article Title: Foxp3 and Toll-like receptor signaling balance T reg cell anabolic metabolism for suppression

doi: 10.1038/ni.3577

Figure Lengend Snippet: Foxp3 reduces aerobic glycolysis and promotes mitochondrial oxidative metabolism. (a) Gene-expression microarray analysis and pathway analysis (with the DAVID bioinformatics database) of RNA from primary mouse CD4+CD25− T cells stimulated and transduced with control or Foxp3-expressing retrovirus, presented as enrichment for selected pathways (left and right margins) among genes upregulated (top) or downregulated (bottom) by Foxp3. (b–g) Immunoblot analysis (b,c), glucose uptake (d), glycolysis (as ECAR after glucose injection) (e) basal OCR (f) and basal OCR/ECAR (g) of primary mouse CD4+CD25− T cells stimulated and transduced with control (NGFR) or Foxp3-expressing retrovirus. Numbers above lanes (b,c) indicate band intensity relative to that of actin. CPM, counts per minute. *P < 0.05 (Two-tailed Student’s t-test). Data are representative of an experiment with biological triplicates for each group (a), three independent experiments (b,c) or two independent experiments with technical triplicates (d–g; mean + s.d.).

Article Snippet: The cDNA was used in the Glucose Metabolism and T cell Anergy and Immune Tolerance SuperArray RT 2 Profiler PCR arrays according to the manufacturer’s instructions and assayed on a ViiA 7 (Applied Biosystems).

Techniques: Gene Expression, Microarray, Transduction, Control, Expressing, Western Blot, Injection, Two Tailed Test

Journal: Nature immunology

Article Title: Foxp3 and Toll-like receptor signaling balance T reg cell anabolic metabolism for suppression

doi: 10.1038/ni.3577

Figure Lengend Snippet: Foxp3 expression is sufficient to suppress glycolysis and promote oxidative metabolism. (a–i) Glucose uptake (a), glycolytic rate (assessed by a radiolabeled-glucose-tracer assay) (b), ECAR (c), flux of glucose into the pentose phosphate pathway (PPP) (d), OCR (e), OCR/ECAR (f), pyruvate oxidation (g), fatty-acid oxidation of palmitate (h) and glutamine oxidation (i) of vector-control-transduced FL5.12 pro-B cells or FL5.12 pro-B cells transduced with tamoxifen-inducible expression of Foxp3, treated with 4-hydroxytamoxifen. (j) Quantification of live cells as in a–i to assess cell accumulation over time. *P < 0.05 (two-tailed Student’s t test). Data are representative of three (a,b,j) or two (c–i) independent experiments with three independent clones per group (mean + s.d. in a–i or (mean ± s.d. in j).

Article Snippet: The cDNA was used in the Glucose Metabolism and T cell Anergy and Immune Tolerance SuperArray RT 2 Profiler PCR arrays according to the manufacturer’s instructions and assayed on a ViiA 7 (Applied Biosystems).

Techniques: Expressing, Plasmid Preparation, Control, Transduction, Two Tailed Test, Clone Assay